It always makes a difference if you are a 2 or 3 until the last team standing is determined. Nothing with respect to who you got paired with changes at anytime after the pairings are made. To think otherwise is just foolish. If you went in as a 2, theoretically only one team in the regional is better than you. If you went in as a three, theoretically two teams in the regionals are better then you. You play the games to determine if the theoretical rankings hold out. 1, 2, 3 or 4 - you play to win the game.